Functional and Highly Perfused Tissue-Engineered Construct for Ginigival Tissue Regeneration using Degradable/Polar/Hydrophobic/Ionic Polyurethanes and Dynamic Co-Culture System
Supervisor: Dr. J. Paul Santerre
Periodontal diseases affect over 40% of Americans 20 years and older. In treatments aimed at gingival tissue regeneration, human gingival fibroblasts (HGF) contribute to the repair of the lamina propria and mediate epithelial cell morphogenesis. The objective of this study is to regenerate gingival tissue using tissue engineering and a dynamic culture system with fibroblasts and vascular endothelial cells. We hypothesize that this technique will produce a functional, highly perfused tissue-engineered construct which physically and biologically models important characteristics of the natural lamina propria. Previous studies using D-PHI scaffolds showed that the material is not cytotoxic; it inhibits inflammation post- implantation and may support the adhesion and growth of an HGF cell line. Current studies include improving HGF proliferation rate using media perfusion as well as characterization of these cells in dynamic culture. Supported by: NSERC CGS D3, CellSignals Training Fellowship (TGF-53877).
Keywords: Fibroblasts, Polyurethanes, Collagen
Jane Cheung
Contact Information
- University of Toronto
Department of Institute of Biomaterials and Biomedical Engineering - 124 Edward Street,
Toronto, Ontario,
Canada M5G1G6 - Email: jane.cheung@utoronto.ca
- Work: 416-979-4903 ext 4349
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