Transmembrane Pressure Differentials as a Biophysical Basis for Air-Liquid Interface Differentiation
Supervisor: Christopher Moraes
This project explores an innovative approach to improve air-liquid interface (ALI) techniques, crucial for creating realistic tissue and organ models in drug development and medical research. The study introduces a novel prototype device that applies transepithelial hydrostatic pressure to cell cultures, aiming to address the limitations of traditional ALI methods, such as lengthy culture times and handling difficulties. By comparing the effects of this new method on primary lung cells and intestinal organoids with those of conventional ALI conditions, preliminary results indicate enhanced differentiation, reduced culture times, and new insights into ALI differentiation mechanics. This research could significantly advance drug development and medical science by offering a more efficient and reliable means of generating tissue models, potentially leading to quicker discoveries and more effective treatments.
Keywords: Air-liquid interface, Epithelial cells, Differentiation
Equipment: Transwell culture
Techniques: Cell culture
Chen Li
Contact Information
- McGill University
Department of Chemical Engineering - 1414 Rue Chomedey,
Montreal, Quebec, H3H 0A2 - Email: shinee9clee@gmail.com
- Home: 5145889866
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