Canadian Biomaterials Society
La Société Canadienne des Biomatériaux

Fingerprinting of Proteins that Mediate Quagga Mussel Adhesion using a De Novo Assembled Foot Transcriptome

Journal: Scientific Reports
Authors: Angelico Obille - David J. Rees, Arash Hanifi, Angelico Obille, Robert Alexander & Eli D. Sone

The European freshwater mollusk Dreissena bugensis (quagga mussel), an invasive species to North America, adheres to surfaces underwater via the byssus: a non-living protein ‘anchor’. In spite of its importance as a biofouling species, the sequence of the majority of byssal proteins responsible for adhesion are not known, and little genomic data is available. to determine protein sequence information, we utilized next-generation RNA sequencing and de novo assembly to construct a cDNA library of the quagga mussel foot transcriptome, which contains over 200,000 transcripts. Quagga mussel byssal proteins were extracted from freshly induced secretions and analyzed using LC-Ms/Ms; peptide spectra were matched to the transcriptome to fingerprint the entire protein primary sequences. We present the full sequences of fourteen novel quagga mussel byssal proteins, named Dreissena bugensis foot proteins 4 to 17 (Dbfp4–Dbfp17), and new sequence data for two previously observed byssal proteins Dbfp1 and Dbfp2. Theoretical masses of the newly discovered proteins range from 4.3 kDa to 21.6 kDa. These protein sequences are unique but contain features similar to glue proteins from other species, including a high degree of polymorphism, proteins with repeated peptide motifs, disordered protein structure, and block structures.

Year: 2019

Volume: 9

Angelico Obille

Contact Information

  • University of Toronto
    Department of Institute of Biomedical Engineering
  • 170 College Street, Room 308A,
    Toronto, Ontario, M5S 3E3
  • Email: angelico.obille@mail.utoronto.ca
  • Home: 6478033670
  • Membership#S222432

Follow us on Twitter @CanBiomatSoc

Biomaterial Highlight Of The Month

Osteoblastic cell interactions with nanoporous titanium surfaces

Marianne Ariganello



Read More

Close Drag