Glucose oxidase kinetics using MnO2 nanosheets: confirming Michaelis–Menten kinetics and quantifying decreasing enzyme performance with increasing buffer concentration
Journal: Nanoscale Advances
Authors: Ungku Zoe Anysa Ungku Faiz - Mahip Singh, Steven Gravelsins, Yoshinori Suganuma, Nicholas Konstantine Kotoulas, Mark Croxall, Ahlia Khan-Trottier, Cynthia Goh, and Al-Amin Dhirani
GOx kinetics is consistent with the Michaelis–Menten (MM) model, and reveals that the MM constant increases by an order of magnitude with increasing buffer concentration. Since larger MM constants imply higher enzyme substrate concentrations are required to achieve the same rate of product formation, increasing MM constants imply decreasing enzyme performance.
Year: 2021
Volume: 3
PP: 3816
Ungku Zoe Anysa Ungku Faiz
Contact Information
- University of Toronto
Department of Chemistry and MSE - 209 Beverley St,
Toronto, Ontario, M5T1Z4 - Email: ungkuzoe@gmail.com
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